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MR imaging-based cerebral perfusion metrics can be obtained by tracing a contrast bolus through the brain microvasculature. The authors compared the calculated resting relative perfusion metrics obtained from the choroid plexus (CP) with those obtained from the middle cerebral artery (MCA) in healthy participants and patients with glioma. The findings of this study suggest that an arterial input function chosen from within the CP is comparable with one chosen from the MCA and may be an alternative, particularly when there is no suitable MCA location to interrogate.