Article Figures & Data
Figures
- FIG 1.
Gross photographs of human blood clot analogs pre- and postfixation. Gross photographs of human blood clot analogs of various compositions were obtained pre- and postfixation in 10% neutral buffered formalin. Clot analogs were created using different ratios (1:5, 1:10, 1:50, and 1:100) of RBC+buffy coat and plasma only, platelets only, and plasma + platelets.
- FIG 2.
Histologic quantification of MSB-stained human clots retrieved from patients and human clot analogs created in vitro. A, The histologic composition of 100 various clots retrieved from patients was assessed using the MSB stain and grouped according to their dominant component, resulting in 3 main groups; RBC-rich, fibrin-rich, and platelet-rich. B, The histologic composition of various human clot analogs created in vitro was also assessed using the MSB stain and grouped according to their dominant component, resulting in the same 3 phenotypes; RBC-rich, fibrin-rich, and platelet-rich.
- FIG 3.
Histology and immunohistochemical-stained images of clots retrieved from a patient with AIS and of a human clot analog. A comparison of the similarity in terms of gross photographs, MSB stain, CD42b (platelets), and vWF between a clot retrieved from a patient with AIS (A) and a human clot analog (1:50 RBC + buffy coat/platelets) is shown (B). MSB stain: RBCs (yellow), WBCs (purple), fibrin (red), platelets/other (grey), and collagen (blue). Platelets (CD42b) and vWF; positive staining is red.
Tables
Ratio Platelets (µl) Plasma(µl) RBC + Buffy Coat(µl) Plasma only 1:5 2400 600 1:10 0 2700 300 1:50 0 2940 60 1:100 0 2970 30 Platelets only 1:5 2400 0 600 1:10 2700 0 300 1:50 2940 0 60 1:100 2970 0 30 Plasma and platelets 1:5 1200 1200 600 1:10 1350 1350 300 1:50 1470 1470 60 1:100 1485 1485 30 ↵a Three microliters of thrombin (1 NIH/mL) and 300 µL of 5% CaCl2 were added to stimulate coagulation.
Clot Type Ingestion Rate FPE Rate SR Rate Passes to Achieve SR DE Rate Soft DA 80% 100% 100% 1.0 0 STR + A NA 100% 100% 1.0 0 Elastic DA 40% 100% 100% 1.0 20% STR + A NA 40% 100% 1.8 60% Stiff DA 20% 40% 40% 1.0 80% STR + A NA 60% 80% 1.3 40% Note:—DE indicates distal embolization; STR + A, stent retriever + aspiration; NA, not applicable.
